Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Alternative backbones? Check iGEM 2013
- Actual list of experiments
- TAL14 & GAL4 (to test promoter)
- MAV1212-hEF1a-eBFP2
- Make/Find primers
- To sequence both low sensors
miRNA Sensors (Lab Work)
- Sequence/digest plasmids to verify - may be unnecessary. Emailed Brian.
- EF
- GH - don't have this
- pEXPR: MAV1212-Hef11a-eBFP2
- Mutant Hef1a-2xKturn-eGFP
- Wild Type Hef1a-2xKturn-eGFP
- Pick colonies and grow up
- MAV1212-mRFP
- L1_TALER14_L2
- pTAL14-mKate-pEST
- MAV1212-Hef1a-eBFP2 for midiprep (pending Brian's approval)
- EF low sensor for midiprep (pending Brian's approval)
- Mutant Hef1a-2xKturn-eGFP for midiprep (pending Brian's approval)
- WT Hef1a-2xKturn-eGFP for midiprep (pending Brian's approval)
- Miniprep
- GH - don't have this
- Transformations
- GH (Golden Gate)
- Hef1a-L7ae (LR)
- Hef1a-TAL14 (LR)
- BP
- P4-donor-P1R with TAL14-mKate pEST
NOTES
BP rxn didn't yield colonies --> re-doing it and letting it grow overnight this time