Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Troubleshoot
- Why didn't the oligos nanodrop??
- Meet w/ Kyle about FlowJo
miRNA Sensors (Lab Work)
- Golden Gate
- Low Sensor miR-144-miR-181c
- Pick colonies
- High Sensor miR-30d
- High Sensor miR-146a
- High Sensor let-7f
- High Sensor miR-125b
- Low Sensor miR-144
- Low Sensor miR-181c
- pENTR: TAL14
- pENTR: TAL21
- pENTR: Something for yeast for Brian
- Restriction Digest & run gel
- pEXPR: MAV1212-TRE-L7ae (LR)
- pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)
- pEXPR: MAV1212-TRE-GAL4VP16 (LR)
- Inoculate midiprep culture?
- If the LR's have the right sequence
- Gateway
- pEXPR: MAV1212-hEF1a-TAL14
- FACS Prep
NOTES
The gel didn't work very well, so we're re-doing it tomorrow