Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
- Meet w/ Kyle
- FlowJo
- Bright Field Images
miRNA Sensors (Theory)
- Troubleshoot
- What's going on with the double input low sensor
- Run new transfection plate/protocol by Brian
- Move siRNA into our cryobox
- Grab sequencing primer from Jeremy's bench
miRNA Sensors (Lab Work)
- Miniprep
- pEXPR: MAV1212-hEF1a-TAL14
- Restriction Digest & run gel (4 samples)
- pEXPR: MAV1212-TRE-L7ae (LR)
- Transform
- pEXPR: MAV1212-TRE-GAL4VP16 (LR)
- All the low sensors
- Transfect for L7ae Experiment 1.3
NOTES
We transfected way too much DNA --> new protocol with 1 uL total