Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
- Meet w/ Kyle
- FlowJo
- Bright Field Images
miRNA Sensors (Theory)
- Troubleshoot
- What's going on with the double input low sensor
- What's the deal with MAV1212: hEF1a-GAL4VP16 (midi???)
miRNA Sensors (Lab Work)
- Digest and Run Gel
- pEXPR: MAV1212-hEF1a-TAL14
- Inoculate midi prep culture
- pEXPR: MAV1212-hEF1a-TAL14
- LR
- pEXPR: MAV1212-TRE-L7ae (again since the digest failed repeatedly??)
- Pick colonies (even from sensor plates)
- pEXPR: MAV1212-TRE-GAL4VP16 (LR)
- Low Sensor miR-144
- Low Sensor miR-181c
- High Sensor miR-30d
- High Sensor miR-125b
- High Sensor miR-146a
- High Sensor let-7f
- Make new Amp/Xgal plates
- Re-transform (give to treatment group)
- Low Sensor miR-144
- Low Sensor miR-181c
- High Sensor miR-30d
- High Sensor miR-125b
- High Sensor miR-146a
- High Sensor let-7f
- Split & Seed for L7ae experiment take 3
NOTES
We ran out of Lipofectamine and couldn't transfect