miRNA To Do List

Long Term Goals

miRNA Profile

• Read literature
• Choose profile

Building the Cell Detector Circuit

• Build mathematical model
• Make test bed
  • Controlled miRNA expression
  • Constructed miRNA sensors

Mini experiments

• • Single miRNA detector circuit

Alzheimer's Model

• Agree on a team wide, full Alzheimer's model
  

Today's Agenda

How To Please Ron/Brain

• Keep updating the parts tree (include experiments)
• Meet w/ Kyle
  • FlowJo
  • Bright Field Images

miRNA Sensors (Theory)

• Troubleshoot
  • What's going on with the double input low sensor
  • What's the deal with MAV1212: hEF1a-GAL4VP16 (midi???)

miRNA Sensors (Lab Work)

• Digest and Run Gel
  
  • pEXPR: MAV1212-hEF1a-TAL14
• Inoculate midi prep culture (if digest works)
  • pEXPR: MAV1212-hEF1a-TAL14
• LR 
  
  • pEXPR: MAV1212-TRE-L7ae (again since the digest failed repeatedly??)
• Pick colonies (even from sensor plates)
  
  • pEXPR: MAV1212-TRE-GAL4VP16 (LR)
  • Low Sensor miR-144
  • Low Sensor miR-181c
  • High Sensor miR-30d
  • High Sensor miR-125b
  • High Sensor miR-146a
  • High Sensor let-7f
• Make new Amp/Xgal plates
• Transform (give to treatment group)
  • Low Sensor miR-144
  • Low Sensor miR-181c
  • High Sensor miR-30d
  • High Sensor miR-125b
  • High Sensor miR-146a
  • High Sensor let-7f
  • pEXPR: MAV1212-hEF1a-GAL4VP16
• Split & Seed for L7ae experiment take 3
• Dilute MAV1212-TAL14-mKate to 500 ng/uL and move to TC room
• Re-dilute double input low sensor target sites
• Golden Gate
  • Low Sensor miR-144-181c
    
    

NOTES