Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
- Meet w/ Kyle
- FlowJo
- Bright Field Images
miRNA Sensors (Theory)
- Troubleshoot
miRNA Sensors (Lab Work)
- Midi prep
- pEXPR: MAV1212-hEF1a-TAL14
- Transform
- pEXPR: MAV1212-TRE-L7ae
- Proteinase K
- MAV1212-hEF1a-TAL14
- Restriction Digest and run gel
- MAV1212-hEF1a-TAL14
- MAV1212-hEF1a-TAL14
- LR
- MAV1212-hEF1a-TAL14
- MAV1212-hEF1a-TAL21
- Mini prep
- Low Sensor miR-144
- Low Sensor miR-181c
- High Sensor miR-30d
- High Sensor miR-125b
- High Sensor miR-146a
- High Sensor let-7f
- Send for sequencing
- Low Sensor miR-144
- Low Sensor miR-181c
- High Sensor miR-30d
- High Sensor miR-125b
- High Sensor miR-146a
- High Sensor let-7f
- Pick colonies
- Low Sensor miR-144
- Low Sensor miR-181c
- High Sensor miR-30d
- High Sensor miR-125b
- High Sensor miR-146a
- High Sensor let-7f
- pEXPR: MAV1212-hEF1a-GAL4VP16
- pEXPR: MAV1212-TRE-GAL4VP16
- Seed plates
- L7ae experiment (Take 3)
- TAL14 experiment (Take 1)
NOTES