Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Troubleshoot
- What's the deal with the GAL4VP16?!
miRNA Sensors (Lab Work)
- Pick colonies
- pEXPR: MAV1212-TRE-L7ae (mini)
- MAV1212-hEF1a-GAL4VP16 (midi)
- MAV1212-TRE-GAL4VP16 (midi)
- Proteinase K
- MAV1212-hEF1a-TAL21
- MAV1212-hEF1a-TAL14
- Transform
- MAV1212-hEF1a-TAL21 (LR)
- MAV1212-hEF1a-TAL14 (LR)
- Some plasmids from BCR group
- Miniprep
- All the sensors (round 2)
- Send for sequencing
- All the sensors (round 2)
- Analyze sequencing results
- All the sensors (round 1)
- Transfect
- L7ae Experiment (Take 3)
- Seed
- TAL14 Experiment (Take 1)
- Re-anneal
- miR-144-181c double input target site
- Golden Gate
- Low Sensor miR-144-181c
- Inoculate midi cultures
- Each of the single input sensors
NOTES
All of the single input sensors were verified!