To do today:
- Run DNA for gel extraction. Also look to see if other, verified DNA contaminated or not.
- Pick colonies fusions
- Transform
Tomorrow:
- Miniprep lots of fusions. Make cell stocks. Verify, get rid of extra stocks.
- pick colonies for pEXPR_hEF1a: LilrB2
Monday:
- Midiprep pEXPR_hEF1a: LilrB2 again. Make cell stock. THROW AWAY ALL OTHER STOCKS.
Is it worth doing the gel extraction? No.
Labeling is pretty bad. L23, L24, L33, L34 are verified. L2s and L3s are monoclonal. I don't know which one the Midiprep DNA came from. But given it came from one of these, no point in gel extracting. Confusion is bad.
DNA running:
HL, L23, L24, L33, L34, Midiprep.