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To Do:

Get LRs to work

  • Transform all LRs (hEF1a:PT, hEF1a:LT, hEF1a:TC, TREt:TC)
  • Pick hEF1a:YC colonies
  • Miniprep TREt:YC if it worked. Otherwise, wait for Brian.
  • Test LilrB2 digest in random places. Is the main LilrB2 stock missing a chunk?

 

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