What experiments are we planning to do with the neurons? 

  • beta amyloid assay with app/appswe transfection about that..
    • we decided against thioflavin t because it binds to beta amyloid fibrils and we don't know whether or not it will bind oligomers
    • we think beta amyloid antibodies are probably our best option 
    • doing an ELISA assay is probably another option 
  • action potentials with and without beta amyloid production
    • how long will it take 
  • miRNA testing? 
  • capstone/prelims in neurons? 

 

What neuron options do we have?

  • brainbits neurons
  • french sebastien neurons have channel rhodopsin so can fire and turn pretty colors
    • (but we have to differentiate those)
    • can we differentiate them into not motor neurons (more representative of alzheimer's disease)
    • we should talk to sebastien about how he manipulates his neurons/ if he's willing to give us some already differentiated cells
  • sebastien palacio's neurons - "talk to eldi" sebastien
    • not rhodopsin-y --> not cool  
    • not motor neurons - more accurate representation of alzheimer's 
    • this sebastien is more likely to help us work with the neurons/ address any problems that we face

What do we need to know about the neurons? 

  • how are they transfected?
  • how long do they last?
  • how long do they take to differentiate?
  • No labels