Long Term Goals
Building the Cell Detector Circuit
- Choose profile
- Verify sensors
- Create mathematical model
Experiments
- L7ae repression
- TAL14 repression
- TAL21 repression
- Single input sensors
- Multiple input sensors
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Emal Jake Beal about TASBE tools
- Pick up sensors from Jeremy
miRNA Sensors (Lab Work)
- Tissue Culture
- Transfect plate for miRNA sensors w/ siRNA
- Miniprep
- hEF1a-TAL14
- (Restriction Digest and Run Gel)
- hEF1a-TAL14
- (Golden Gate)
- TAL14 High Sensors
NOTES