Long Term Goals
Building the Cell Detector Circuit
- Choose profile
- Verify sensors
- Create mathematical model
Experiments
- L7ae repression
- TAL14 repression
- TAL21 repression
- Single input sensors
- Multiple input sensors
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Emal Jake Beal about TASBE tools
- Pick up sensors from Jeremy
- And figure out what/when to do with them...
- New map for siRNA experiments
- Re-name siRNAs as "siRNA-[insert number here]"
- Figure out scatterplot in MATLAB
miRNA Sensors (Lab Work)
- Tissue Culture
- Cytometry for miRNA sensors w/o siRNA
- Golden Gate
- TAL14 High Sensors
- Grow in midi culture
- Double input low sensor
- GG Donor for TC
- hEF1a-TAL21
NOTES