4/2 Meeting Notes

Safety - get trained
PRIORITY: decide which system
- 1st system: Hydrogen production from cellulose by co-culture of Clostridium thermocellum JN4 and Thermoanaerobacterium thermosaccharolyticum GD17
  - http://www.sciencedirect.com/science/article/pii/S0360319908003844
- 2nd system: Phototrophic hydrogen production from glucose by pure and co-cultures of Clostridium butyricum and Rhodobacter sphaeroides
  - http://www.sciencedirect.com/science/article/pii/S0360319906001170
- Read the papers on each system
Next meeting moved from Thurs (4/9) to Sunday (4/12) at 3 pm because of 8.02 exam
Project Structure
- Metabolism - measuring rate of glucose and lactate production
  - C. Therm:
    - rate lactate production
    - lactate production vs cellulose concentration
  - T. Therm: H2 production vs lactate concentration
    - instantaneous
    - batch
    - continuous culture
- Signaling - AutoInducer-2
  - Make sure the bacteria are transformable
  - Make sure we can send our parts back to iGEM headquarters at end of the summer
  - Senders send:
    - chromatography
  - Receivers receive:
    - AI-2 -> GFP
    - [GFP] vs [AI-2]