| Construct | F Tm | R Tm | Rxn T |
|---|---|---|---|
| CD79A | 61 | 60 | 63 |
| CD79B | 66 | 67 | 69 |
| Gal4VP16 | 66 | 62 | 65 |
| pTEt:mRFP | 51 | 58 | 61 |
| Construct | Expected Band Size |
|---|---|
| CD79A | 750 bp |
| CD79B | 750 bp |
| Gal4VP16 | 750 bp |
| pTet:mRFP | 800 bp |
| Ladder | CD79A | CD79B | Gal4VP16 | pTet:mRFP |
 |  |
Notes:
CD79A and CD79B bands are not of the correct size. They appear to have primer dimers. Temperature used for the PCR may not be appropriate, since we changed PCR reagents from Phusion to Q5.
Gal4VP16 and pTet:mRFP construct might be correct - too much DNA in the lane to discern exact position of the band.
PCRs will be redone with correct temperatures and less template.