CD79B PCR with High GC Fusion:
Construct | F Tm | R Tm | Rxn T |
|---|---|---|---|
| CD79B | 69.1 | 73 | 72 |
Gel:
| Ladder | Gel | Notes |
|---|---|---|
 |  | wtf? |
pTet:mRFP and Gal4VP16 PCR Purification:
| Construct | Yield |
|---|---|
| pTet:mRFP | 48.7 ng/uL |
| Gal4VP16 | 46.7 ng/uL |
CD79A Gel Extraction:
| Construct | Yield |
|---|---|
| CD79A | 19 ng/uL |
Golden Gate:
hEF1a:CD79A-pTet:mRFP-Gal4VP16