Chicken Lysozyme
Chicken lysozyme derives from chicken egg white. Breaks down bacterial cell walls.
mAb name: HyHEL-10
Peptide suppliers:
Sigma-Aldrich: 1g/$49.50
Merck-Millipore: 1g/$39
HIV1 gp120 (HIV envelope glycoprotein)
gp120 is a glycoprotein derived from gp160. gp160 is processed into gp120 and gp41, which together form a complex that binds to CD4, permitting the fusion of viral and cell membranes.
mAb database: HIV Molecular Immunology 2002 (same as this)
mAb name: 0.5beta
Sequence: IMGT
Epitope (recognition sequence):
IMGT: RKSIRIQRGPGRAFVTIG
HIV molecular immunology database: RGPGRAFVTIG
Peptide suppliers:
Full-length gp120 peptides are not readily available. Check the manufacturing details to ensure that the epitope is included in the peptide. Also check that peptide comes glycosylated.
Abcam: 10ug/$228
MyBioSource: 50ug/$365
**PROBLEM: These have the amino acid sequence RKRIRIQRGPGRAFVTIG
Tried testing binding in silico:
Performed in silico mutation using Swiss PDB Viewer: S234R, where 234 is PDB residue number (contained in peptide P) corresponding to S residue in RKSIRIQRGPGRAFVTIG
Energy minimization with Python code from 20.320 using PyRosetta.
But, looking more closely at PDB file SPV didn't add hydrogens with the mutation 