Colonies Picked
Re-transformed Constructs (No colonies from previous transformation)
hEF1a:CD79B-TCS-Gal4VP16
hEF1a:Lyn
TRE:Lyn-TEV
Sequencing
hEF1a:CD79A-pTet:RFP-Gal4VP16 sent for sequencing
PCR
Used primers CD79B F3 Tm = 59 and CD79B R3 Tm = 59
Annealed at 62C
Expected Band Size 750 BP
Gel
| CD79B | Hyperladder | Notes: It is extremely strange that the PCR has four different bands. This may be due to a degenerate base in the reverse primer and the forward primer's affinity for AarI sites. The lowest band appears to be 750 BP and a gel extraction will be performed to recover DNA from that band. |
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Gel