CD79B PCR Gel
Left: Ladder, Right: ~750bp band corresponding to CD79B and a primer dimer smear as expected from modeling - Gel Extraction Performed to remove the dimersCD79B PCR Gel Extraction
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14.8ng/uL yield
hEF1a:CD79B-pTet:RFP-Gal4VP16 Golden Gate
Used fragments CD79B, Gal4VP16, and RFP, with hEF1a:CD79B-TCS-Gal4VP16 as the GG Donr vector
Lyn Colonies Picked
Small colonies and generally slow growth happened with Lyn-TEV which likely resulted in the failed 5mL culture on 05/30 5 cultures were inoculated with different colonies to improve chances of success