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06/16:

LR

06/18

transformation

06/19

pick colonies

06/22

miniprep

06/23

digest + gel

potential mix up in loading the gel, diges+gel repeated

06/25

digest + gel

reLR

06/29

digest + gel of LR1 with different enzymes

cultured and miniprep LR2


Gel

6/23

Notes:

Inconclusive - could potentially be a failed LR with extra bands corresponding to a partial digestion. We were concerned that gel may have been loaded in an order different to what was recorded

6/25

Notes:

Band pattern doesn't match either failed or successful LR. could potentially be a partial digestion with the top band at around 7kb (the size of the plasmid) and the bottom two bands being the correct digestion.

Correct.


 

Sequencing results

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