hEF1a:CD79B-pTet:mRFP-Gal4VP16

PCR: when we redesigned primers to fix dimerization issues, we accidentally introduced a missense mutation in the third codon from the end of CD79B. We decided to use SDM to fix the mutation instead of rePCRing

SDM primers arrived 06/19

 

hEF1a:CD79B-XnTCSXc-Gal4VP16

3TCS3 9TCS3 15TCS3
     
 6TCS69TCS612TCS6 
     
3TCS96TCS99TCS912TCS915TCS9
     
 6TCS129TCS1212TCS12 
     
3TCS15 9TCS15 15TCS15
     
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