| TRE:Syk-15-tTEVp | EGSH:mKate | hEf1a:VgEcR-2A-RXR | TRE:mKate | hEF1a:Gal4VP16 | hEF1a:CD79B-TCS-Gal4VP16 | hEF1a:CD79A-3T3-Gal4VP16 | hEF1a:CD79A-6T6-Gal4VP16 | hEF1a:CD79A-6T12-Gal4VP16 | hEF1a:CD79A-9T3-Gal4VP16 | |
|---|---|---|---|---|---|---|---|---|---|---|
| Enzyme | StuI, ApaI Cuts in TRE, Syk, tTEVp and backbone | StuI cuts in mKate and backbone | ApaI Cuts in hEF1a, VgEcR-2A-RxR and bacbone | StuI cuts in TRE, mKate, and backbone | XhoI cuts in hEF1a, Gal4VP16, and backbone | PvuII Cuts in CD79B, TCS and backbone | PvuII Cuts in CD79A, TCS and backbone | PvuII Cuts in CD79A, TCS and backbone | PvuII Cuts in CD79A, TCS and backbone | PvuII Cuts in CD79A, TCS and backbone |
| digest tube/lane | 21,22,23,24 | 1,2,3,4 | 13,14,15,16 | 17,18,19,20 | 5,6,7,8 | 9,10,11,12 | 25 | 26 | 27 | 28 |
| Virtual gel |
Actual failure:
|  |  |  |  |  |  | | | |
 |
well 7 wasn't loaded |
| DNA construct | Constants Definition | ladder | EGSH:mKate | EGSH:mKate | EGSH:mKate | EGSH:mKate | hef1a:vp16-gal4 | hef1a:vp16-gal4 | hef1a:vp16-gal4 | hef1a:vp16-gal4 | hef1a:cd79b-tcs-g4 | hef1a:cd79b-tcs-g4 | hef1a:cd79b-tcs-g4 | hef1a:cd79b-tcs-g4 | hef1a:VgEcR-2a-RxR | hef1a:VgEcR-2a-RxR | hef1a:VgEcR-2a-RxR | hef1a:VgEcR-2a-RxR | TRE:Mkate | TRE:Mkate | ladder | ladder | TRE:Mkate | TRE:Mkate | Tre:syk-15aa-tTevp | Tre:syk-15aa-tTevp | Tre:syk-15aa-tTevp | Tre:syk-15aa-tTevp | he1a:CD79a-3t3-g4 | he1a:CD79a-3t3-g5 | he1a:CD79a-3t3-g6 | he1a:CD79a-3t3-g7 | ladder |
| Tube identifier | n/a | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | 1 | 2 | n/a | n/a | 3 | 4 | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | n/a | |
| DNA concentration (ng/ul) | n/a | 5.6 | 35 | 74.5 | 191.6 | 5.4 | 9.7 | 120.3 | 198 | 45.1 | 60.3 | 156.6 | 80.3 | 11.6 | 17.7 | 247.8 | 248.4 | 36.3 | 49.5 | n/a | n/a | 131.4 | 189.4 | 131.1 | 12 | 169.7 | 177.1 | 40.1 | 48.2 | 18.9 | 46.9 | n/a | |
| Enzyme Name | n/a | StuI | StuI | StuI | StuI | XhoI | XhoI | XhoI | XhoI | PvuII | PvuII | PvuII | PvuII | ApaI | ApaI | ApaI | ApaI | StuI | StuI | n/a | n/a | StuI | StuI | StuI+ApaI | StuI+ApaI | StuI+ApaI | StuI+ApaI | PvuII | PvuII | PvuII | PvuII | n/a | |
| Buffer Name | n/a | cs | cs | cs | cs | cs | cs | cs | cs | 3.1 | 3.1 | 3.1 | 3.1 | cs | cs | cs | cs | cs | cs | n/a | n/a | cs | cs | cs | cs | cs | cs | 3.1 | 3.1 | 3.1 | 3.1 | n/a | |
| gel/lane | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 |
| strip tube # | 1/1 | 1/2 | 1/3 | 1/4 | 1/5 | 1/6 | 1/7 | 1/8 | 1/9 | 1/10 | 1/11 | 1/12 | 2/1 | 2/2 | 2/3 | 2/4 | 2/5 | 2/6 | 2/7 | 2/8 | 2/9 | 2/10 | 2/11 | 2/12 | 3/1 | 3/2 | 3/3 | 3/4 | 3/5 | 3/6 | 3/7 | 3/8 | |
| results | No | No | No | Correct | No | No | Correct | Correct | No | No | Correct | No | No | No | Correct | Correct | Correct | Correct | Correct | Correct | No | No | No | No | No | No | No | No |