| Construct | EGSH:mKate | hEF1a:CD79B-TCS-G4 | hEF1a:Gal4VP16 | hEF1a:tagBFP | hEF1a:VgEcR-2A-RxR | TRE:mKate | hEF1a:CD79A-XTX-Gal4VP16 |
|---|---|---|---|---|---|---|---|
| type | Midi | Midi | Midi | Midi | Midi | Midi | Mini |
| enzyme | StuI cuts in mKate and backbone | PvuII Cuts in CD79B, TCS and backbone | XhoI cuts in hEF1a, Gal4VP16, and backbone | StuI Cuts in hEF1a, tagBFP and backbone | ApaI Cuts in hEF1a, VgEcR-2A-RxR and bacbone | StuI cuts in TRE, mKate, and backbone | NcoI |
| tube/lane | 1 | 2 | 3 | 4 | 5 | 6 | 7-24 |
| virtual gel |  |  |  |  |  |  |  |
| 1-6 | 7-23 |
|---|---|
| 3T3, 3T9, 3T15...
|
