Construct | pENTR HyHEL-10 Light | pENTR Syk-15-tTEV | CD79A 12t12 #1 mini | CD79A3t3 midi | hEF1a:VP16Gal4 midi | hEF1a:VP16Gal4 midi | hEF1a:VP16Gal4 midi |
|---|---|---|---|---|---|---|---|
| Enzyme | BsaXI | BsaXI | NcoI | NcoI | undigested control | XhoI | StuI |
| Tube/Lane | 1,2 | 3,4 | 5 | 6 | 7 | 8 | 9 |
| Virtual gel |  |  |  | |  |  |
| DNA construct | Constants Definition | pENTR HyHEL-10 Light | pENTR HyHEL-10 Light | pENTR Syk-15-tTEV | pENTR Syk-15-tTEV | CD79A-12T12-gal4 | CD79A-12T9-gal4 | hef1a:vp16-gal4 | hef1a:vp16-gal4 | hef1a:vp16-gal4 |
| Tube identifier | 1 | 2 | 1 | 2 | 1 | MIDI | ||||
| gel lane | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
| DNA mass desired (ng) | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 |
| total volume(ul) | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| DNA concentration (ng/ul) | 67.3 | 63.6 | 229.4 | 176.5 | 170 | 459.8 | ||||
| Enzyme Name | BsaXI | BsaXI | BsaXI | BsaXI | NcoI | NcoI | undigested | XhoI | StuI | |
| Buffer Name | cs | cs | cs | cs | cs | cs | ||||
| gel/lane | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
| DNA volume | 7.43 | 7.86 | 2.18 | 2.83 | 2.94 | 1.09 | #DIV/0! | #DIV/0! | #DIV/0! | |
| buffer volume | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
| enzyme volume | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| strip tube # | 1/1 | 1/2 | 1/3 | 1/4 | 1/5 | 1/6 | 1/7 | 1/8 | 1/9 | |
| water volume | 17.0 | 9.6 | 9.1 | 14.8 | 14.2 | 14.1 | 15.9 | #DIV/0! | #DIV/0! | #DIV/0! |
| gel/lane | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
| loading dye | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
| results | ||||||||||
| notes | ||||||||||
| virtual gel |
