TRE:Syk-15-tTEVp Mini | TRE:Syk-6-tTEVp Midi | ||
|---|---|---|---|
| Enzyme | EcoRI | EcoRI | |
| Tube/Lane | 1, 2 | 3 | |
| Virtual Gel |  |  |
| DNA construct | Constants Definition | TRE:Syk-15-tTEV | TRE:Syk-15-tTEV | TRE:Syk-6-tTEV |
| Tube identifier | 1 | 2 | MIDI | |
| DNA concentration (ng/ul) | 158.9 | 250.4 | 599.8 | |
| Enzyme Name | EcoRI-hf | EcoRI-hf | EcoRI-hf | |
| Buffer Name | CS | CS | CS | |
| strip tube # | 1/1 | 1/2 | 1/3 | |
| water volume | 17.0 | 13.9 | 15.0 | 16.2 |
| DNA volume | 3.15 | 2.00 | 0.83 | |
| buffer volume | 2 | 2 | 2 | 2 |
| enzyme volume | 1 | 1 | 1 | 1 |
| loading dye | 4 | 4 | 4 | 4 |
| DNA mass desired (ng) | 500 | 500 | 500 | 500 |
| total volume(ul) | 20 | 20 | 20 | 20 |
| gel/lane | #REF! | 1 | 2 | 3 |
| results | ||||
| notes | ||||
| virtual gel |