hEF1a:CD79B-XnTCSXc-Gal4VP16

hEF1a:CD79B-pTet:mRFP-Gal4VP16

PCR: when we redesigned primers to fix dimerization issues, we accidentally introduced a missense mutation in the third codon from the end of CD79B. We decided to use SDM to fix the mutation instead of rePCRing

06/19

• SDM

06/23

• Gel verification of SDM --> no bands: possible that there wasn't enough amplification. will run SDM again and transform product directly 

06/24

• SDM and DpnI digest 
• transform 

06/25

• No colonies --> could be because a small amount of the digest was transformed.
• all of digest transformed

06/26

• No colonies

hEF1a:CD79B-XnTCSXc-Gal4VP16