1. Use the cryogenic (freezer) tubes to make your freezer stock.
2. Add 1.5 mL of your over night culture (fresh) to 345 uL of 80% glycerol.
3. Make sure that the box you put your tubes in to can handle the low temperature (-80C).
1. Use the cryogenic (freezer) tubes to make your freezer stock.
2. Add 1.5 mL of your over night culture (fresh) to 345 uL of 80% glycerol.
3. Make sure that the box you put your tubes in to can handle the low temperature (-80C).