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For priority:  1 = top priority (absolutely necessary), 2 = middle priority (would be useful), 3 = low priority (only if we have time)

 

Name conventions

pENTR L1-Gene name-L2

pEXPR Promoter:Gene

NameModuleStatusTypeInitialsConstruction DateLinkExperimentPriority
pEXPR Gal4UAS:eYFPantibodyin someone else's freezerexpression     
pEXPR Gal4UAS:mKateantibodyin someone else's freezer

expression

     
pEXPR Tre-TEVpantibodyin someone else's freezerexpression     
Hef1a-rtTaantibodyin someone else's freezerexpression     
CD79A WTantibodyminiprepped, awaiting sequence verification      
CD79B WTantibodyminiprepped, awaiting sequence verification      
CD79A-TCS-Gal4VP16antibodyplanned      
CD79B-TCS-Gal4VP16antibodyplanned      
Lyn WTantibodygrowing in LB, ready at 7am      
Lyn-eYFPantibodyplanned      
Lyn point mutated (always phosphorylate)antibodyresearching      

Syk WT

antibodygrowing in LB, ready at 7am      
Syk-eYFP (mutant non phosphorylate)antibodyplanned      
Syk-TEVpantibodyplanned      
GMab kantibodyon order      
GMab Hantibodyon order      
GMabH-TCS-Gal4VP16antibodyplanned      
hsa-miR-181cmiRNAplanned      
hsa-miR-9miRNAplanned      
hsa-miR-30d-5pmiRNAplanned      
hsa-miR-144-5pmiRNAplanned      
hsa-let-7f-5pmiRNAplanned      
has-miR-125bmiRNAplanned      
has-miR-146amiRNAplanned      
pEXPR TRE-t:BACE2-eYFPtreatmentplanned      
    • pENTR L4--TRE-t--R1
treatmentin the lab already      
    • pENTR L1--BACE2-eYFP--L2
treatmentplanned      
      • BACE2
treatmentordered      
      • eYFP
treatmentin the lab already      
    • pDEST R4--R2
treatmentin the lab already      
pEXPR Hef1a:BACE1-eYFPtreatmentplanned      
    • pENTR L4--Hef1a--R1
treatmentin the lab already      
    • pENTR L1--BACE1-eYFP--L2
treatmentplanned      
      • BACE1
treatmentto be ordered      
      • eYFP
treatmentin the lab already      
    • pDEST R4--R2
treatmentin the lab already      
pEXPR U6:mKate-miRNA(intronic)treatmentplanned      
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic)treatmentplanned      

 

 

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