| People to Contact | Questions | Contacted Yet? | |
|---|---|---|---|
Jacob Beal | Email sent | ||
Jeremy Gam | Questions | Had first meeting |
Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
Presentations
- Run through NEGEM Presentation
- Logic version of high/low sensor circuits
miRNA Sensors (Theory)
- Create circuit diagram (Raymond)
- Put it on our subgroup home page
- Determine final output of circuit
- How does repressor affect the circuit?
- Determine our repressors and repressible promoters (use ones available in existing backbone)
- Understand why we're doing any of these reactions
miRNA Sensors (Lab Work)
- Transform MAV1212 into ccdB resistance cells
- Grow at low temp w/ x-gal, shielded from light
- Pick as many colonies as possible
- Sequence ccdB region
- Propogate plasmids (in room 215, in -20 fridge, labeled "igem 2014")
- MAV1212
- MAV1212-Hef1a-EBFP2
- (MAV1212-Hef1a-mKate2)
- Gateway MAV1212-Hef1a-L7ae
- Find Hef1a-L7ae
Cell Models
- Finish table of concentrations in each cell line
- Finalize siRNA transfection technique
Social Media
- Public wiki
- Paragraph from sponsorship booklet
- Facebook presence