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For priority:  1 = top priority (absolutely necessary), 2 = middle priority (would be useful), 3 = low priority (only if we have time)

 

Name conventions

pENTR L1-Gene name-L2

pEXPR Promoter:Gene

Status

WordMeaning
in planningbeing designed in geneious
designedplasmid design completed in geneious
in labsomeone else in the lab has it
ordered 
in construction 
constructed 
in storagewe have it in our fridge/freezer, etc
NAMEGROUPSTATUSTYPELOCATIONINITIALSCONSTRUCTED ONOBTAINED FROMPRIORITYNOTES
pEXPR hEF1a: Gmab Heavy         
pEXPR hEF1a: Gmab Light         
pEXPR hEF1a: CD79A         
pEXPR hEF1a: CD79B         
          
          
          
          
          
          
pEXPR Gal4UAS:eYFPAntibodyin someone else's freezerexpression      
pEXPR Gal4UAS:mKateAntibodyin someone else's freezer

expression

      
pEXPR Tre-TEVpAntibodyin someone else's freezerexpression      
Hef1a-rtTaAntibodyin someone else's freezerexpression      
CD79A WTAntibodypossibly mislabled DNA and frozen stock DNA in freezer, frozen bacteria stock in -80     
CD79B WTAntibodyminiprepped, awaiting sequence verification DNA in freezer, frozen bacteria stock in -80     
CD79A-TCS-Gal4VP16Antibodyplanned       
CD79B-TCS-Gal4VP16Antibodyplanned       
Lyn WTAntibodyminiprepped, need to nanodrop and sequence DNA in freezer, frozen bacteria stock in -80     
Lyn-eYFPAntibodyplanned       
Lyn point mutated (always phosphorylate)Antibodyresearching       

Syk WT

Antibodyminiprepped, need to nanodrop and sequence DNA in freezer, frozen bacteria stock in -80     
Syk-eYFP (mutant non phosphorylate)Antibodyplanned       
Syk-TEVpAntibodyplanned       
GMab kAntibodyon order       
GMab HAntibodyon order       
GMabH-TCS-Gal4VP16Antibodyplanned       
hsa-miR-181cmiRNAplanned       
hsa-miR-9miRNAplanned       
hsa-miR-30d-5pmiRNAplanned       
hsa-miR-144-5pmiRNAplanned       
hsa-let-7f-5pmiRNAplanned       
has-miR-125bmiRNAplanned       
has-miR-146amiRNAplanned       
pEXPR TRE-t:BACE2-eYFPTreatmentplanned       
    • pENTR L4--TRE-t--R1
Treatmentin the lab already       
    • pENTR L1--BACE2-eYFP--L2
Treatmentplanned       
Treatmentin -80plasmid AL    
      • eYFP
Treatmentin the lab already       
    • pDEST R4--R2
Treatmentin the lab already       
pEXPR Hef1a:BACE1-eYFPTreatmentplanned       
    • pENTR L4--Hef1a--R1
Treatmentin the lab already       
    • pENTR L1--BACE1-eYFP--L2
Treatmentplanned       
      • BACE1
Treatmentto be ordered       
      • eYFP
Treatmentin the lab already       
    • pDEST R4--R2
Treatmentin the lab already       
pEXPR U6:mKate-miRNA(intronic)Treatmentplanned       
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic)Treatmentplanned       
Experiment 1         
  • ggDONR
Receptor in storageplasmid+4 in iGEM roomLA- Kyle not sure if blue/white selection is working or not; conducted troubleshooting experiment - problem was with plates; however not sure if concentration is high enough
  • ggDONR New
Receptor in storageplasmid+4 in iGEM roomLA-Brandon will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stock
  • pENTR L4-Hef1a-R1
Receptor in labplasmid     will be used to make LilrB2 expression vector
  • pDEST
Receptor in labplasmid     will be used to make LilrB2 expression vector
  • pEXPR Hef1a:LilrB2
Receptor in constructionplasmid     waiting on entry vector
    • LilrB2 Entry Vector
Receptor in constructionplasmid     ifailing to get liquid cultures to grow in order to miniprep to extract plasmid DNA
      • LilrB2 gBlock
Receptor in storagegBlock-20 in iGEM roomLA- IDT used in Golden Gate
  • PirB Expression Vector
Receptor in construction      waiting on entry vector
    • PirB Entry Vector
Receptor in construction      failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA
      • PirB gBlock C terminus
Receptor in storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate
      • PirB gBlock N terminus
Receptor in storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate
          
          
          

 

 

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