To Do List

Long Term Goals

miRNA Profile

• Read literature
• Choose profile

Building the Cell Detector Circuit

• Build mathematical model
• Make test bed
  • Controlled miRNA expression
  • Constructed miRNA sensors

Mini experiments

• • Single miRNA detector circuit

Alzheimer's Model

• Agree on a team wide, full Alzheimer's model
  

Today's Agenda

How To Please Ron/Brain

• AD blood cells from PrecisionMed? Talk to Brian?
• Meet with Kyle about designing siRNA (email sent)
  • Make siRNA sequences in geneious
  • Send order to Brian
• Picture of how low sensors come together

miRNA Sensors (Theory)

• Determine which pairs of target sites we want
  • Find sequences (hopefully under 200 bp)
  • Send ultramer order to Brian
• Plan new MAV vector
• Plan high sensor construction (meet with Brian)

miRNA Sensors (Lab Work)

• Golden Gate
  • Gather miRNA target sites from Jeremy
    • Nanodrop for concentrations ( ? )
  • Make all 6 single low sensors (in conjunction with antibody group)
• Miniprep eBFP2
• Pick colonies from mKate2 and L7ae

NOTES

Both LR's failed (mKate2 and L7ae)