Transformation
Transformed Golden Gate products for Lyn+stop, Lyn-mKate, Lyn-TEVp, Syk-eYFP, Syk-TEVp into ultra competent cells. Plated 200uL of each at 5pm. Removed plates from 37C incubator at 9am.
Liquid culture
Started liquid cultures (5mL LB + 5uL kanamycin) for CD79A+stop, CD79A-TCS-Gal4VP16, Syk+stop. Placed in shaking incubator (37C) at 4:50pm. Removed from incubator to refrigerator at 9am.