• PirB PCR bands extracted 
    • Concentrations very low 
      • around 5 ng/ul
  • PirB PCRs run again 
  • LilrB2 PCRs done at higher annealing temperatures 
  • Cofilin plasmid colonies picked 
  • PirB pEXPRs miniprepped 
  • Restriction digest on PirB pEXPRs run 
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