- PirB LR colonies picked and liquid cultures prepared
- LilrB2 PCRs repeated under 2 different (higher) annealing temperatures
- Remaining PirB PCR product ran and band with the correct size DNA to be extracted
- Gel with PCR products imaged
- Expected sizes:
- LilrB2: 2,000
- PirB: 2,500
HL LilrB2 (YFP) LilrB2 (TCS) PirB (YFP) PirB (TCS) 
- Expected sizes:
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