Date | Description |
---|---|
2014 June 22 | PCRs for CD79A+stop, CD79A-TCS, linker+mKate |
2014 June 24 | DpnI digest for mKate and eYFP |
2014 June 26 | DpnI digest for TEVp Transformations of Golden Gates (CD79A+stop, CD79A-TCS-Gal4VP16, Syk+stop) Golden Gates (Lyn+stop, Lyn-mKate, Syk-eYFP, Lyn-TEV, Syk-TEV) |
2014 June 27 | Picking colonies (CD79A+stop, CD79A-TCS-Gal4VP16, Syk+stop) pUC19 competence |
2014 June 29 | Picking colonies (Lyn+stop, Lyn-mKate, Lyn-TEVp, Syk-eYFP, Syk-TEVp) |
2014 June 30 |