How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
- More descriptive names
- Include other plasmid
miRNA Sensors (Theory)
- Alternative backbones? Check iGEM 2013
- Plan experiment to test Kturn (Just Kturn and L7ae)
- Re-make double input low sensor as two ultramers (use the Q3 site)
miRNA Sensors (Lab Work)
- Pick colonies & grow in medium (pick 5)
- Midi prep
- EF
- GH
- pEXPR: Hef1a-eBFP2
- Mutant Hef1a-2xKturn-eGFP
- Wild Type Hef1a-2xKturn-eGFP
- Mini prep
- pENTR: L7ae
- GH
- Midi prep
- Mini prep
- EF
- pEXPR: Hef1a-eBFP2
- Mutant Hef1a-2xKturn-eGFP
- Wild Type Hef1a-2xKturn-eGFP
- LR
- MAV1212-Hef1a-L7ae
- MAV1212-Hef1a-TAL14
- BP
- P4-donor-P1R on Brian's bench
- TAL14-mKate pEST in Brian's fridge
- Golden Gate
- GH
- Transformation
- TAL14 Promoter (from BP rxn)
- TAL14-mKate-pest
- pENTR: L1_TAL14_L2
- MAV1212-mRFP
NOTES
We should sequence the low sensors when they're done