Cloning Strategy

CD79A Linker Optimization

Antibody Choices 

Friday's to do's

•  Midis
  •  B12T12-2
•  Minis, sequencing
  •  T:S-9-tT
  •  T:S-12-tT
•  Inoculate mini
  •  B15T15
  •  B6T12
  •  B6T9
•  Restreak
  •  B15T9
  •  B12T9
  •  B9T9 (retransform?)
•  Sequencing
  •  B9T15-2
  •  B3T15-1
  •  T:S9tT 1
  •  T:S12tT 1

Thursday's to do's 

•  Midis:
  •  B9T12
  •  B15T3
  •  S9TEV
  •  S12TEV
•  Minis, sequencing 
  •  B6T9
  •  A9T15
•  Inoculate mini
  •  B15T9
  •  B15T15
  •  B12T9
  •  T:S-9-tT
  •  T:S-12-tT
•  Inoculate midi:
  •  B12T12-2
•  Sequencing:
  •  B6T9-1
  •  A9T15-2
  •  A3T15-2
  •  T:S-3-T 1
  •  T:S-6-T-1
•  Restreak:
  •  B15T9
  •  B12T9

Wednesday's to do's 

•  Midis:
  •  B9T12
  •  B15T3
  •  S9TEV
  •  S12TEV
  •  B12T12-2
    
•  Minis, sequencing:
  •  B15T9
  •  B15T15
  •  B12T9
  •  T:S-9-tT
  •  T:S-12-tT
•  Split cells 

Sequencing account information:
^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

Check on status:

• B9T12 - send for seq
• B6T9 - send for seq
• B12T9 - repeat seq
• B12T12 - repeat seq

Midi's currently frozen:

• B9T12 (-80)
• B15T3 (-80)
• S9TEV (-80)
• S12TEV (-80)
• B12T12 (-20)

Non-Lab To Do's 

•  Cytometry time 
•  Order Cumate 
•  Chicken lysozyme, gp120
•  Antibodies for antigen binding detection

Waiting on primers to do list:

Higher Level To Do's:

•  Decide on other antigen-variable regions we want to test  
•  Is gantenerumab proprietary and can we actually publish with it?

THINGS TO NOT FORGET TO DO:

• SYK-15tTEVP golden gate when we figure out what's wrong with the AarI
• 3T3 MIDI