BCR 2.0
Switchable Antigen-Sensing System (S.A.S.S.)
| Center |
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Friday's to
To do's
- Midis
- B12T12-2
- Minis, sequencing
- T:S-9-tT
- T:S-12-tT
- Inoculate mini
- B15T15
- B6T12
- B6T9
- Restreak
- B15T9
- B12T9
- B9T9 (retransform?)
- Sequencing
- B9T15-2
- B3T15-1
- T:S9tT 1
- T:S12tT 1
Thursday's to do's
- Midis:
- B9T12
- B15T3
- S9TEV
- S12TEV
- Minis, sequencing
- B6T9
- A9T15
- Inoculate mini
- B15T9
- B15T15
- B12T9
- T:S-9-tT
- T:S-12-tT
- Inoculate midi:
- B12T12-2
- Sequencing:
- B6T9-1
- A9T15-2
- A3T15-2
- T:S-3-T 1
- T:S-6-T-1
- Restreak:
- B15T9
- B12T9
Wednesday's to do's
- Midis:
- B9T12
- B15T3
- S9TEV
- S12TEV
- B12T12-2
- Minis, sequencing:
- B15T9
- B15T15
- B12T9
- T:S-9-tT
- T:S-12-tT
- Split cells
:
- Midiprep (from freezer)
- S9TEV
- B15T15
- Golden Gate
- B9T9
- B15T9
- LR
- hEF1a-CuO:Gmab H
- hEF1a-CuO:Gmab L
- hEF1a-CuO:CD79A
- hEF1a-CuO:CD79B
- hEF1a-CuO:Lyn
- Digest all of the Syk-TEVp and Syk-tTEVp midis just to be sure (since there's an internal cut site that didn't get read in sequencing)
Sequencing account information:
^^^Email: bcrpeeps@mit.edu
^^^Pass: grapefruit
## Digest tTEVp and TEVp midis just to be sure (since there's an internal cut site)
Check on status:
- B9T12 - send for seq
- B6T9 - send for seq
- B12T9 - repeat seq
- B12T12 - repeat seq
Midi's currently frozen:
- B9T12 (-80)
- B15T3 (-80)
- S9TEV (-80)
- S12TEV (-80)
- B12T12 (-20)
Non-Lab To Do's
- Cytometry time
- Order Cumate
- Chicken lysozyme, gp120
- Antibodies for antigen binding detection
Waiting on primers to do list:
- Bug Brian about ordering antibody testing supplies
Higher Level To Do's:
- Decide on other antigen-variable regions we want to test
- Is gantenerumab proprietary and can we actually publish with it?
THINGS TO NOT FORGET TO DO:
- SYK-15tTEVP golden gate when we figure out what's wrong with the AarI
- 3T3 MIDI