Cloning Strategy

CD79A Linker Optimization

Antibody Choices 

STATUS TABLE

 To do's:

•  Retransform B15T9
•  B9T9 minipreps of cultures that are already in the fridge Midiprep (from freezer)
  •  S9TEV
  •  B15T15
•  Get aliquot of pENTR VP16Gal4 from Jeremy and start LR Golden Gate
  •  B9T9
  •  B15T9
•  LR
  •  hEF1a-CuO:Gmab H
  •  hEF1a-CuO:Gmab L
  •  hEF1a-CuO:CD79A
  •  hEF1a-CuO:CD79B
  •  hEF1a-CuO:Lyn
•  Digest all of the Syk-TEVp and Syk-tTEVp midis just to be sure (since there's an internal cut site that didn't get read in sequencing)

Sunday's to do's

•  Split cells

Saturday's to do's

•  Replate B15T9

Friday's to do's

•  Take B15T15 midiprep out of incubator
•  Take B9T9 colonies out of incubator

Thursday's to do's

•  Replate B15T9
•  Take midipreps out of incubator
•  Reinoculate B15T15 midi
•  Inoculate more B9T9 colonies

Wednesday's to do's

•  Split cells
•  Golden gate/transform B15T9
•  Midiprep
  •  A15T9
  •  B3T15
  •  B9T15
  •  B12T9
•  Inoculate midiprep culture
  •  S9TEV
  •  B15T15

Sequencing account information:
^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

## Digest tTEVp and TEVp midis just to be sure (since there's an internal cut site)

Non-Lab To Do's 

•  Bug Brian about ordering antibody testing supplies

Higher Level To Do's:

•  Decide on other antigen-variable regions we want to test  
•  Is gantenerumab proprietary and can we actually publish with it?