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Zulu Time All.
14-6-17
| Propagating MAV1212 and MAV1212-Hef1a-EBFP2 |
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Stock Concentrations: EBFP2: 1118 ng/µL MAV: unknown. 1856: Diluted DNA samples for both x100 before transformation 4 Plates, LO HI for both. 2144: Culture plates incubated overnight at 30°C for 16-18 hours. |
14-6-18
| Propagating MAV1212 and MAV1212-Hef1a-EBFP2 |
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| 1426: Checked cell plates. EBFP2 produced colonies. MAV1212 overgrown. |
| MAV1212 Streaking | EBFP2 Pick Colonies |
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2044: Picked single colonies from each plate and streaked over xgal plates.
Let on the incubator overnight at 30°C for 16-18 hours. | 2040: Picked one colony each from the HI and LO plates and immersed in LB Media.
Left on the shaker incubator overnight. |
14-6-19
| MAV1212 Streaking |
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| 1254: Disaster. No colonies. |
| Propagating MAV1212 and MAV1212-Hef1a-EBFP2 (Again) |
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Concentrations: EBFP2: 12 ng/µL MAV: 4 ng/µL 4 Plates, LO HI for both. 1715: Culture plates inseerted into 30°C incubator. |