| People to Contact | Questions | Contacted Yet? | |
|---|---|---|---|
Jacob Beal | Had first meeting | ||
Jeremy Gam | Questions | In close contact |
Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Create a clear workflow
- Update "status" of plasmids on parts list
miRNA Sensors (Theory)
- Determine which pairs of target sites we want
- Find sequences (hopefully under 200 bp)
- Send order to Brian to order the ultramer
miRNA Sensors (Lab Work)
- Tranform LRs
- MAV1212-Hef1a-mKate2
- MAV1212-Hef1a-L7ae
- Hef1a on Kan
- Pick colonies from transformations
- MAV1212-Hef1a-eBFP2
- Golden Gate
- Gather miRNA target sites from Jeremy
NOTES
MAV1212 didn't grow (ccdB resistance is over expressed)
Hef1a was plated on Amp plates...but it has Kan resistance
Verify eBFP2