To Do List

Long Term Goals

miRNA Profile

• Read literature
• Choose profile

Building the Cell Detector Circuit

• Build mathematical model
• Make test bed
  • Controlled miRNA expression
  • Constructed miRNA sensors

Mini experiments

• • Single miRNA detector circuit

Alzheimer's Model

• Agree on a team wide, full Alzheimer's model
  

Today's Agenda

How To Please Ron/Brain

• Create a clear workflow
• Update "status" of plasmids on parts list
• How do we string multiple low sensors together?

miRNA Sensors (Theory)

• Determine which pairs of target sites we want
  • Find sequences (hopefully under 200 bp)
  • Send ultramer order to Brian

miRNA Sensors (Lab Work)

• Tranform LRs 
  • MAV1212-Hef1a-mKate2
  • MAV1212-Hef1a-L7ae
  • Hef1a on Kan
• Pick colonies from transformations
  • MAV1212-Hef1a-eBFP2
• Golden Gate
  • Gather miRNA target sites from Jeremy
  • Make all 6 single low sensors

NOTES

MAV1212 didn't grow (ccdB resistance is over expressed)

Hef1a was plated on Amp plates...but it has Kan resistance

Verify MAV1212-Hef1a-eBFP2 via sequencing AmpR/eBFP2 region