BCR 2.0

Cloning Strategy

Linker Optimization

Antibody Choices 

Immediate To Do's:

• kill TRE:Gal4VP16 LR
  
• digests and gels for CD79A linker constructs
• run SDM on gel -> gel extraction
  
• send CD79A-XTCSX-G4 for sequencing 
• transform TRE:Gal4VP16 LR
• transform CD79B-RFP-Gal4 SDM product 

BCR 2.0 To Do

^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

Higher Level To Do's:

• Decide on other antigen-variable regions we want to test  
• Other experimental planning (on experimental page)
• Parts list (excel sheet detailing specs/locations of parts)
  
• How do we do site directed mutagenesis?
• Literature research: 
  • kozak sequences for heavy and light chains and CD79s
  • truncated TEVp
  • other antigens to detect using BCR (we were thinking of small molecule detection)
  • inducible promoters (we were considering LacI - an inducible repressor - but we want to look into other options first) 
• Cloning planning 
  • hEF1a:Syk-pTet:mRFP-TEVp
  • variable region