BCR 2.0

Cloning Strategy

CD79A Linker Optimization

Antibody Choices 

Monday's To Do's 

• Oligomerize AB
• Miniprep, digest, gel
  •  CD79A linker constructs
  • Midis
• transform IgM-RFP golden gate
• golden gate TRE:Syk-15-TEVp in gg donr

Tuesday's To Do's

• Gel for oligo annealing
  • Order new annealing buffer
• Pick RFP-IgM Colony
• Syk-15-TEV GG

Saturday's To Do's 

• Midipreps 
• CD79A golden gate T4 ligation
• Transformations
  • CD79A golden gates 
  • IgM RFP golden gate
• Sequencing
  • 3T3
  • 6T6
  • 6T12
  • 9T3
    Sequencing account information
    
    

^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

Non-Lab To Do's 

• Cytometry time 
• Order Cumate 
• Chicken lysozyme, gp120
• Antibodies for antigen binding detection

Waiting on primers to do list:

• TRE:Syk-15-TEVp
• CD79B-mRFP-G4 PCR

Higher Level To Do's:

• Decide on other antigen-variable regions we want to test  
• Is gantenerumab proprietary and can we actually publish with it?