BCR 2.0

Cloning Strategy

CD79A Linker Optimization

Antibody Choices 

Wednesday's to Dos

• Redigest basically everything from yesterday and gel
• Pick colonies
  • TRE:Syk-15-tTEV
  • hEF1a:CD79B-RFP-G4
  • pENTR VRC01 heavy
  • pENTR VRC01 light
  • pENTR HyHEL-10 heavy
  • pENTR HyHEL-10 light
• Fix
  • TRE:Syk-15-tTEV
  • hEF1a:CD79B-RFP-G4
  • Syk linkers
• Miniprep andn digest
  • CD79A-3T15
  • CD79A-12T9
  • CD79A-15T9
• Inoculate midipreps if the sequencing comes back good

Sequencing account information:
^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

Non-Lab To Do's 

• Cytometry time 
• Order Cumate 
• Chicken lysozyme, gp120
• Antibodies for antigen binding detection

Waiting on primers to do list:

Higher Level To Do's:

• Decide on other antigen-variable regions we want to test  
• Is gantenerumab proprietary and can we actually publish with it?

THINGS TO NOT FORGET TO DO:

• SYK-15tTEVP golden gate when we figure out what's wrong with the AarI
• 3T3 MIDI