BCR 2.0

Cloning Strategy

CD79A Linker Optimization

Antibody Choices 

Tuesday's To Do's 

• Gel TRE:Syk-15-tTEVP -> send for sequencing 
• digest, gel, pENTR HyHEL10 H -> send for sequencing 
• innoculate midi:
  • B15T3
  • T:S-15-tT

• inoculate mini:
  • B15T9 - from mini culture
  • B9T9 - from plate
  • B15T15 - from plate
  • A3T15 - from plate 
  • A9T15 - from plate 

Sequencing account information:
^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

Check on status:

• B9T12 - send for seq
• B6T9 - send for seq
• B12T9 - repeat seq
• B12T12 - repeat seq

Non-Lab To Do's 

• Cytometry time 
• Order Cumate 
• Chicken lysozyme, gp120
• Antibodies for antigen binding detection

Waiting on primers to do list:

Higher Level To Do's:

• Decide on other antigen-variable regions we want to test  
• Is gantenerumab proprietary and can we actually publish with it?

THINGS TO NOT FORGET TO DO:

• SYK-15tTEVP golden gate when we figure out what's wrong with the AarI
• 3T3 MIDI