PCR of Lyn-(15aa)

Gel shows successful PCR of Lyn-(15aa) from pENTR Lyn (pDONR223).

Forward primer:  iGEM CR 006 F

Reverse primer:  iGEM_AS009 Lyn Qlonglink_R

Annealing Temperature:  60C

Run using samples after PCR purification.

PCR of TEVp

Gel shows successful PCR of TEVp from pENTR_L1_Tev2.

Forward primer:  iGEM_AS010 TEVp Qlonglink_F

Reverse primer:  iGEM_AS011 TEVp QX_R

Annealing Temperature:  60C

Run using samples after PCR purification.

Restriction Digest Verification of pENTR

1 July 2014:

See gel here

3 July 2014:

See gel here

7 July 2014:

See gel here