PCR of Lyn-mKate

Gel shows successful PCR of Lyn-mKate from pENTR Lyn (pDONR223).

Forward primer:  iGEM CR 006 F

Reverse primer:  iGEM KRB 001 R

Annealing Temperature:  60C

Run using samples after PCR purification.

PCR of mKate

Gel shows successful PCR of linker+mKate.

Forward primer:  iGEM KRB 006 F

Reverse primer:  iGEM KRB 007 R

Annealing temperature:  60C

Run using samples before PCR purification.

PCR purified and restriction digest run with DpnI.

Restriction Digest Verification of pENTR

1 July 2014:

See gel here

3 July 2014:

See gel here

7 July 2014:

See gel here