PCR of Syk-eYFP (no DMSO)

Gel shows unsuccessful PCR of Syk-eYFP from pENTR Syk (pDONR223).

Forward primer:  iGEM_AS004 Syk Q1_F

Reverse primer:  iGEM KRB 010 R

Annealing Temperature:  55C

Run using samples after PCR purification.

PCR of Syk-eYFP (5% DMSO)

Gel shows successful PCR of Syk-eYFP from pENTR Syk (pDONR223).

Forward primer:  iGEM_AS004 Syk Q1_F

Reverse primer:  iGEM KRB 010 R

Annealing Temperature:  52C?

Run using samples after PCR purification.

PCR of eYFP

Gel shows successful PCR of eYFP from pENTR_L1_EYFP_L2.

Forward primer:  iGEM KRB 008 F

Reverse primer:  iGEM KRB 009 R

Annealing Temperature:  55C

Run using samples after PCR purification.

Restriction Digest Verification of pENTR

1 July 2014:

See gel here

3 July 2014:

See gel here

7 July 2014:

See gel here