BCR 2.0
Experimental Progress:
Cloning Progress
Parts Tree
Cloning Strategy
Experiments
Linker Optimization
Antibody Choices
Useful Links:
Protocols
Immediate To Do's:
| Task | Claimed by | Date Completed | Other Notes |
|---|---|---|---|
| CD79A/B, Gal4VP16, RFP PCR try3 | CR | Apr 10 |
|
| Gel verification of try3 PCR product | LA | Apr 10 | RFP and Gal4 worked, CD79s didn't - we probably need to redesign primers |
| Redesign primers | LA + CR | Apr 11 | CD79A F and R and CD79B F primers were redesigned and ordered |
| AarI golden gate of CD79A construct | LA | Apr 30 | |
| Take out CD79A golden gate plates | LA | May 2 | |
Make LB | CR | May 4 | |
CD79A golden gate liquid cultures | LA | May 4 | Cultures in at 6pm - need to be taken out by 10/11 am May 5 in 215 shaking incubator |
| Take out CD79A golden gate liquid cultures | LA | ||
| |||
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| Redesign CD79B reverse PCR primer |
- task dependent on above task
Higher Level To Do's:
- Finish Discussing Figures for Experiment list
- Decide on other antigen-variable regions we want to test
- Syk-Lyn experiment design
- Receptor dilution experiment design
- Other experimental planning