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BCR 2.0

 

Cloning Progress

Experiments

Parts Tree

Parts Library

Timeline

Cloning Workflow

Protocols

 

 

Cloning Strategy
CD79A Linker Optimization
Antibody Choices 

 

Monday's To Do's 
  • Oligomerize beta-amyloid
  • Midi's:
  • digest:
  • Make Sodium Azide solution 
  • Pick colonies:
    • TRE:Syk-15-tTEVp golden gate
    • Golden gate donor mini product
    • LRs:
      • EGSH:mKate
      • hEf1a:VgEcR-2A-RXR
      • TRE:mKate
      • hEF1a:Gal4VP16 (remember which pDEST)
      • hEF1a:CD79B-TCS-Gal4VP16 
      • hEF1a:tagBFP

Non-Lab To Do's:

  • Order chicken lysozyme and gp120?
  • order antibodies for antigen binding detection


Geneious To Do List:
  • Antibody cloning for chicken lysozyme - Kathryn 
  • hef1a sequencing primersBCR 2.0 To Do

^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

 

 

Higher Level To Do's:
  • Decide on other antigen-variable regions we want to test  
  • Is gantenerumab proprietary and can we actually publish with it?

 

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